(1 000 000 000; short scale: 1 billion; long scale: 1 milliard)
−1, and as such is the largest number which can fit into a signed (two's complement) 32-bit integer on a computer, thus marking the upper computational limit of a 32-bit CPU such as Intel's Pentium-class computer chips.
Lanes 1 and 2, 25 µg of protein from Cos-1 cells transfected with E7 or empty control plasmid, respectively; lanes 3 and 4, 10 and 50 µg of bovine MDBK cell lysate, respectively; lanes 5 and 6, anti-E7 immunoprecipitates from bovine fibropapilloma (lane 5) or uninfected bovine tissue (lane 6).
E5 oncoprotein transmembrane mutants dissociate fibroblast transforming activity from 16-kilodalton protein binding and platelet-derived growth factor receptor binding and phosphorylation.
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