An A1 broth is a liquid culture medium used in microbiology for the detection of fecal coliforms in foods, treated wastewater and seawater bays using the most probable number (MPN) method. It is prepared according to the formulation of Andrews and Presnell given below. It is used with a Durham tube, a positive tube being one that exhibits a trapped bubble of gas.
Suspend the dry ingredients in one liter of cold distilled water. Gently heat until completely dissolved and distribute 9 ml into test tubes with an inverted Durham tube. Sterilize in an autoclave at 121°C for 15 minutes. If needed, prepare multi-strength broth weighing the appropriate quantity of the dry medium. The final pH is 6.9 ± 0.1.
The whole broth and cell-free filtrates were active against Botrytis and Alternaria in in vitro tests and were active against Botrytis in in vivo small plant tests on Astilbe.
The novel metabolite-producing Bacillus subtilis can be provided as a suspension in a whole broth culture or as a metabolite-containing supernatant or a purified metabolite obtained from a whole broth culture of the microorganism.
AQ713 samples tested were whole broth and spray dried powder of the whole broth grown in a 400 liter fermenter in medium 4.
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