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Encyclopedia > APCI
Atmospheric pressure chemical ionization interface

Atmospheric pressure chemical ionization (APCI) is an ionization method used in mass spectrometry. It is a form of chemical ionization which takes place at atmospheric pressure. Mass spectrometry (also known as mass spectroscopy (deprecated)[1] or informally, mass-spec and MS) is an analytical technique used to measure the mass-to-charge ratio of ions. ... Chemical ionization (CI) is an ionization technique used in mass spectrometry. ...


How it works

APCI allows for the high flow rates typical of standard bore HPLC to be used directly, often without diverting the larger fraction of volume to waste. Typically the mobile phase containing eluting analyte is heated above 400 degrees Celsius, sprayed with high flow rates of nitrogen and the entire aerosol cloud is subjected to a corona discharge that creates ions. Often APCI can be performed in a modified ESI source. This is basically a gas phase ionisation unlike ESI, which is a liquid phase ionisation process. Also, we can use nonpolar solvent for solution making instead of polar solvent for supporting ions in solution as gaseous state conversion of solvent before reaching to coron discharge pin is carried out here, which well supports the ions formed. Chromatography is a family of analytical chemistry techniques for the separation of mixtures. ... In electricity, a corona discharge is an electrical discharge brought on by the ionization of a fluid surrounding a conductor, which occurs when the potential gradient exceeds a certain value, in situations where sparking (also known as arcing) is not favoured. ... An electrostatic potential map of the nitrate ion (NO3−). Areas coloured red are lower in energy than areas colored yellow An ion is an atom or group of atoms which have lost or gained one or more electrons, making them negatively or positively charged. ... Electrospray ionization (ESI) is a technique used in mass spectrometry to produce ions. ...


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