An agarose is a polysaccharidepolymer material, generally extracted from seaweed. The molecules are extremely water-soluble due to their large number of hydroxyl groups, and the solutions tend to be low-melting point aqueous gels. Sheets of agarose gels are readily prepared by pouring the warm, liquid solution into a mould, and are frequently used in molecular biology for the separation of large molecules by electrophoresis. A wide range of different agaroses, of varying molecular weights and properties are commercially available for this purpose. Jump to: navigation, search Polysaccharides (sometimes called glycans) are relatively complex carbohydrates. ... Jump to: navigation, search A polymer is a generic term used to describe a substantially long molecule. ... Jump to: navigation, search Seaweed covered rocks in the UK Phycologists consider seaweed to refer any of a large number of marine benthic algae that are multicellular, macrothallic (large-bodied), and thus differentiated from most algae that tend towards microscopic size (Smith, 1944). ... A gel (from the lat. ... Jump to: navigation, search Molecular biology is the study of biology at a molecular level. ... Jump to: navigation, search Digital printout of an agarose gel electrophoresis of cat-insert plasmid DNA Agarose gel electrophoresis is a method used in molecular biology to separate DNA strands by size, and to determine the size of the separated strands by comparison to strands of known length. ...
See also: Agar Jump to: navigation, search Agar is a galactose polymer (or agarose) obtained from the cell walls of some species of red algae or seaweed (Sphaerococcus euchema) and species of Gelidium, chiefly from eastern Asia and California. ...
Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and captures the resultant gel slurry in the sample filter cup.
As the agarose is compressed at 5,000 x g, DNA is extruded from the gel's pores.
Centrifugation forces the agarose through the gel nebulizer, converting it to a fine slurry that is captured by the sample filter cup.