John Stewart, 2nd Duke of Albany (1481 or 1484–2 July 1536 in Mirfleur, France) was Regent of the Kingdom of Scotland, Duke of Albany in peerage of Scotland and count-consort of Auvergne and Lauraguais in France.
Albany was his whole life the next heir of the Kingdom of Scotland after male members of the king's immediate family, due to stipulations of the Semi-Salic succession order enacted by Robert II of Scotland which favored male agnates over all females of the Royal House of Stewart.
Thus Albany was from 1504 onwards either the heir presumptive or the second-in-line to the throne of Kingdom of Scotland.