The patients were all residents of the municipalities of Belém (31,506 samples) and Ananindeua (8,731 samples).
Of all sera collected, 17,525 (43.5%) were positive by serologic testing or DEN virus isolation: 13,805 (78.8%) from Belém and 3,720 (21.2%) cases from Ananindeua (Figure).
During 1953 and 1954, Causey and Theiler (11) used seroneutralization in mice to survey several municipalities of the Amazon region; they found 9.8% and 2.2% of serum samples positive for dengue virus serotypes DEN-1 and DEN-2, respectively, among residents 50 years of age.
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