Anna Valerious was a fictional vampire hunter in the film Van Helsing. She was the Princess of a Gypsy Clan in Romania. Her father was abducted by Dracula in 1887, and was shortly after killed. Prince Velkan, her brother, was bitten by a werewolf while he tried to protect his sister from the werewolf's attack. Anna, thinking that Velkan was dead, later realised he had actualy been transformed into the Wolf Man and unwillingly became a spy of Dracula.

A few weeks later, Van Helsing and Friar Carl came to Transylvania where Anna lives. Van Helsing was sent from Rome to protect her and to help in her family's quest in slaying Dracula. But Anna, who is very bullheaded, didn't want to accept his help. But before she had the chance to formaly object, he village is viciously attacked by the three brides of Dracula's: Aleera, Verona, and Mariska.

Anna, who flees, knowing that the brides have come for her to finish killing off her family, leaves Van Helsing who is fighting Mariska, whom he later stakes to death.

At the end of the movie Anna is killed by Van Helsing while he is the Wolf Man, but since Dracula was killed prior to her death, Anna and the entire Valerious family is freed from Purgatory and is accepted into Heaven since her family's cause, killing Dracula, has been completed. She smiles at Van Helsing, while her body is being burned on the pyre behind him, as he looks into the clouds feeling her presence with him.