Aultsville is a ghost town in the Canadian province of Ontario. It is one of Ontario's Lost Villages, which were permanently flooded by the creation of the St. Lawrence Seaway in 1958. The town was founded as Charlesville in 1787 by United Empire Loyalists. It was the second largest town flooded by the new Seaway. Before its flooding the abandoned buildings were burned to study the effects of fire on houses.

Families and businesses in Aultsville were moved to the new town of Ingleside before the seaway construction commenced. The historic train station was moved to Upper Canada Village where it remains today.