Bovine serum albumin, Bovine Albumin, BSA: A serum albumin protein that can be used as a diluent or a blocking agent in numerous applications including ELISAs (Enzyme-Linked Immunosorbent Assay), blots and immunohistochemistry. It is also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. This protein does not affect other enzymes that do not need it for stabilization. BSA is used because of its stability, its lack of effect in many biochemical reactions, and its low cost since it is readily available in large quantities as it is purified from bovine blood, a byproduct of the beef industry. You may be looking for albumen, or egg white. ... Categories: Move to Wiktionary | Stub ... Elisa (born Elisa Toffoli on 19 December 1977) is an Italian singer and solo artist, writing and performing within several genres, notably rock, blues, soul and ambient. ... Immunohistochemistry or IHC refers to the process of localizing proteins in cells of a tissue section exploiting the principle of antibodies binding specifically to antigens in biological tissues. ... A restriction digest is a molecular biology procedure used to prepare DNA for analysis or other processing. ... The structure of part of a DNA double helix Deoxyribonucleic acid (DNA) is a nucleic acid that contains the genetic instructions for the development and function of living organisms. ... A representation of the 3D structure of myoglobin, showing coloured alpha helices. ... Ribbon diagram of the enzyme TIM, surrounded by the space-filling model of the protein. ...

Physical properties

• Number of residues: 583
• Molecular Weight: 66,430 kDa
• Theoretical pI: 5.82
• Extinction Coefficient: 0.6 ml mg^-1 cm^-1

A 18-residue signal peptide is cut off the precursor protein upon secretion, hence the precursor has 607 amino acid residues and a molecular weight of 69,323.4 kDa. A signal peptide is a short (15-60 amino acids long) peptide chain that directs the post transrational transport of a protein. ...

References

• The sequence of BSA on NCBI.
• Product Sheet Information at Sigma-Aldrich
• Largest manufacturer of BSA: Proliant

Sigma-Aldrich Corporation NASDAQ: SIAL, headquartered in St. ...

See also

• Human serum albumin
• Serum albumin
• PEGylation

It has been suggested that this article or section be merged into serum albumin. ... You may be looking for albumen, or egg white. ... // In 1970s, pioneering research by Davis, Abuchowski and colleagues foresaw the potential of the conjugation of Polyethylene glycol (PEG) to Proteins. ...

External links

• MeSH Serum+Albumin,+Bovine