|
Protein - Wikipedia, the free encyclopedia (5422 words) |
 | The end of the protein with a free carboxyl group is known as the C-terminus or carboxy terminus, while the end with a free amino group is known as the N-terminus or amino terminus. |
| A "tag" consisting of a specific amino acid sequence, usually a series of histidine residues (a "His-tag"), is attached to one terminus of the protein. |
| As a result, when the lysate is passed over a chromatography column containing nickel, the histidine residues ligate the nickel and attach to the column while the untagged components of the lysate pass unimpeded. |
| Mutation of Ha-Ras C Terminus Changes Effector Pathway Utilization -- Booden et al. 275 (31): 23559 -- Journal of ... (7910 words) |
| from that induced by Ha-Ras61L with the native C terminus. |
| of the C terminus of Ext61L to the membrane. |
| To study the role of the C terminus in Ha-Ras signaling, both membrane binding and activity must be retained. |
Feeds |
Contact