| Coomassie |
 | | Identifiers | | CAS number | 6104-58-1 | | PubChem | 6333920 | | SMILES | CCN(CC1=CC(=CC=C1)S(=O) (=O)O)C2=CC(=C(C=C2)C(=C3C=CC(=[N+] (CC)CC4=CC(=CC=C4)S(=O)(=O)O)C=C3C) C5=CC=C(C=C5)NC6=CC=C(C=C6)OCC)C Image File history File links Coomassie. ...
CAS registry numbers are unique numerical identifiers for chemical compounds, polymers, biological sequences, mixtures and alloys. ...
PubChem is a database of chemical molecules. ...
The simplified molecular input line entry specification or SMILES is a specification for unambiguously describing the structure of chemical molecules using short ASCII strings. ...
| | Properties | | Molecular formula | C47H50N3O7S2+ | | Molar mass | 833.048 | Except where noted otherwise, data are given for
materials in their standard state
(at 25 °C, 100 kPa)
Infobox disclaimer and references | Coomassie (also known as Coomassie Blue, Brilliant Blue, Brilliant Blue G, Acid Blue 90, C.I. 42655, Brilliant Blue G 250, or Kunasty Blue) is a blue dye commonly used in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The gel is soaked in dye for thirty minutes and then destained for thirty minutes or more. This treatment allows the visualization of bands indicating the protein content of the gel. The gel usually contains a set of molecular weight marker (proteins of pre-determined weight) so that protein molecular weight can be determined in an unknown solution during the visualization. A chemical formula is a concise way of expressing information about the atoms that constitute a particular chemical compound. ...
Molar mass is the mass of one mole of a chemical element or chemical compound. ...
The plimsoll symbol as used in shipping In chemistry, the standard state of a material is its state at 1 bar (100 kilopascals exactly). ...
Image File history File linksMetadata Download high resolution version (2288x1712, 773 KB) Photographer: Dasha Title: Coomassie Description: Solution of Coomassie, which turns blue if protein is present. ...
Image File history File linksMetadata Download high resolution version (2288x1712, 773 KB) Photographer: Dasha Title: Coomassie Description: Solution of Coomassie, which turns blue if protein is present. ...
Sodium dodecyl sulfate (or sulphate) (SDS or NaDS) (C12H25NaO4S), also known as sodium lauryl sulfate (SLS), is an ionic surfactant that is used in household products such as toothpastes, shampoos, shaving foams and bubble baths for its thickening effect and its ability to create a lather. ...
Polyacrylamide is an acrylate polymer formed from acrylamide subunits that is readily cross-linked. ...
Gel electrophoresis is a technique used for the separation of deoxyribonucleic acid, ribonucleic acid, or protein molecules through an electric charge. ...
Picture of an SDS-PAGE. The molecular marker is in the left lane SDS-PAGE stands for Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis. ...
A representation of the 3D structure of myoglobin, showing coloured alpha helices. ...
The molecular mass of a substance (less accurately called molecular weight and abbreviated as MW) is the mass of one molecule of that substance, relative to the unified atomic mass unit u (equal to 1/12 the mass of one atom of carbon-12). ...
Variations Shown at right is Coomassie G250. A similar but distinct dye, known as
Coomassie R-250, also exists. Image File history File links Coomassie_Brilliant_Blue_R-250. ...
Laboratory usage Coomassie dye is an integral component of the Bradford Method for determining protein concentration in a solution. The Coomassie dye binds to proteins via physisorption[citations needed] to arginine, the aromatic amino acids, and histidine. When Coomassie Brilliant Blue G-250 binds to proteins in acid solution, it has an absorbance shift from 465 nm to 595 nm. The absorbance data can then be used in Beer's law to determine protein concentration and ultimately the actual amount of protein in a given solution. The Bradford Protein Assay is a spectroscopic analytical procedure used to measure the concentration of protein in a solution. ...
Physisorption is a process whereby a molecule adheres to a surface without the formation of a chemical bond, usually by van der Waals forces or electrostatic attraction. ...
Arginine (abbreviated as Arg or R)[1] is an α-amino acid. ...
phenylalanine tryptophan tyrosine thyroxine Aromatic amino acids are amino acids which include an aromatic ring. ...
Histidine is one of the 20 most common natural amino acids present in proteins. ...
In optics, the Beer-Lambert law, also known as Beers law or the Beer-Lambert-Bouguer law is an empirical relationship in relating the absorption of light to the properties of the material the light is travelling through. ...
A representation of the 3D structure of myoglobin, showing coloured alpha helices. ...
Making a saline water solution by dissolving table salt (NaCl) in water This article is about chemical solutions. ...
Coomassie stain and destain solution formulas For staining solution: - 2.50 g Coomassie Brilliant Blue R-250,
- 455 mL ethanol,
- 455 mL deionized / distilled water,
- 90 mL glacial acetic acid
For destaining solution: Grain alcohol redirects here. ...
The chemical compound acetic acid (from the Latin word acetum, meaning vinegar), systematically called ethanoic acid, is the acid that gives vinegar its sour taste. ...
- 455 mL ethanol,
- 455 mL deionized / distilled water,
- 90 mL glacial acetic acid
(Can also be destained using only distilled water and heating, though at a much slower pace)
Common mix for Bradford reagent:
- 0.01% Coomassie Brilliant Blue G-250,
- 4.7% ethanol,
- 8.5% phosphoric acid, in deionized / distilled water.
Methanol has been used in the past and can be replaced by ethanol at all steps resulting in slightly safer reagents (methanol is a neurotoxin) while the efficiency of the procedure remains the same.
Name Like many dyes, Coomassie blue dye is named after an African locale, in this case the city of Kumasi, now in Ghana. It was developed as an acid wool dye and was named to commemorate the 1896 British occupation of the Ashanti capital, then known as Coomassie.[1] Look up dye in Wiktionary, the free dictionary. ...
Kumasi is the capital city of the Ashanti region of Ghana. ...
Ashanti is a administrative region in central Ghana. ...
References is the 41st day of the year in the Gregorian calendar. ...
Year 2007 (MMVII) is the current year, a common year starting on Monday of the Gregorian calendar and the AD/CE era in the 21st century. ...
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