Coomassie blue is a dye that can be used in immunoelectrophoresis. It's a general stain used for proteins Image File history File links Coomassie. ... CAS registry numbers are unique numerical identifiers for chemical compounds, polymers, biological sequences, mixtures and alloys. ... PubChem is a database of chemical molecules. ... Medical Subject Headings (MeSH) is a huge controlled vocabulary (or metadata system) for the purpose of indexing journal articles and books in the life sciences. ... This article or section does not cite any references or sources. ... Molar mass is the mass of one mole of a chemical element or chemical compound. ... In chemistry, the standard state of a material is its state at 1 bar (100 kilopascals) and 25 degrees Celsius (298. ... Look up dye in Wiktionary, the free dictionary. ... Immunoelectrophoresis (IES) is the electrophoresis of a determined antigen mixture in an agarose gel that allows the separation of different proteins along the gel slide, and then the lateral diffusion in the gel of an immune serum or a monoclonal antibody. ... A representation of the 3D structure of myoglobin, showing coloured alpha helices. ...
Coomassieblue binds to proteins in an approximately stoichometric manner, meaning that when the relative amounts of protein need to be assessed, densitometry of the stained protein will provide data.
Other uses for brilliant blue dyes are detection of lipids in thin layer chromatography, for detection of proteins in solution and on cellulose acetate plates, and as a traceable dye for following water uptake in the roots of plants.
To a chemist, brilliant blue is actually a diammonium salt known also as acid blue or a disodium salt, sometimes called alphazurine, featuring 37 carbon atoms, 34 hydrogens, 2 nitrogens, 2 sodiums, 9 oxygens, and 3 sulfurs per molecule.
Unbound CoomassieBlue absorbs light maximally at a wavelength of 465 nm, while the absorption maximum is at 595 nm when the dye is bound to protein.
Coomassieblue (the dye in the Bradford Reagent), phosphoric acid, and methanol are very harmful when swallowed, absorbed through the skin or splashed in the eyes.
Coomassieblue, phosphoric acid, and methanol are components of the Bradford Reagent.
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