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Encyclopedia > Crick, Brenner et al. experiment

The Crick, Brenner et al. experiment was a scientific experiment performed in 1961 by Francis Crick and Sydney Brenner. They demonstrated that three bases of DNA code for one amino acid in the genetic code. The experiment elucidated the nature of gene expression and frameshift mutations. From Latin ex- + -periri (akin to periculum attempt). ... Francis Harry Compton Crick OM FRS (8 June 1916 – 28 July 2004) was an English molecular biologist, physicist, and neuroscientist, who is most noted for being one of the co-discoverers of the structure of the DNA molecule in 1953. ... To meet Wikipedias quality standards, this article or section may require cleanup. ... A series of codons in part of a mRNA molecule. ... Gene expression, or simply expression, is the process by which a genes DNA sequence is converted into functional proteins. ... A frameshift mutation (also called a frameshift or a framing error) is a genetic mutation that inserts or deletes a number of nucleotides that is not evenly divisible by three from a DNA sequence. ...


In the experiment, proflavin-induced mutations of the T4 bacteriophage gene, rIIB, were isolated. Proflavin causes mutations by inserting itself between DNA bases, typically resulting in insertion or deletion of a single base pair. Proflavine (pron. ... It has been suggested that mutant be merged into this article or section. ... Enterobacteria phage T4 is a phage that infects E. coli bacteria. ... A bacteriophage (from bacteria and Greek phagein, to eat) is a virus that infects bacteria. ...


The mutants produced by Crick and Brenner could not produce functional rIIB protein because the insertion or deletion of a single nucleotide caused a frameshift mutation. Mutants with two or four nucleotides inserted or deleted were also nonfunctional. However, the mutant strains could be made functional again by using proflavin to insert or delete a total of three nucleotides. This proved that the genetic code uses a codon of three DNA bases that corresponds to an amino acid. A frameshift mutation (also called a frameshift or a framing error) is a genetic mutation that inserts or deletes a number of nucleotides that is not evenly divisible by three from a DNA sequence. ... RNA codons. ... Phenylalanine is one of the standard amino acids. ...


References

  1. Crick FH, Barnett L, Brenner S, Watts-Tobin RJ. General nature of the genetic code for proteins. Nature. 1961 Dec 30;192:1227-32. PubMed: Entrez PubMed 13882203.

Francis Harry Compton Crick OM FRS (8 June 1916 – 28 July 2004) was an English molecular biologist, physicist, and neuroscientist, who is most noted for being one of the co-discoverers of the structure of the DNA molecule in 1953. ... To meet Wikipedias quality standards, this article or section may require cleanup. ... The Entrez logo The Entrez Global Query Cross-Database Search System allows access to databases at the National Center for Biotechnology Information (NCBI) website. ...

See also


  Results from FactBites:
 
Sydney Brenner - Wikipedia, the free encyclopedia (580 words)
Brenner then turned his sights on establishing Caenorhabditis elegans as a model organism for the investigation of animal development including neural development.
Brenner chose this 1 millimeter-long soil roundworm mainly because it is simple, is easy to grow in bulk populations, and turned out be quite convenient for genetic analysis.
Brenner founded the Molecular Sciences Institute and is currently associated with the Salk Institute and the Institute of Molecular and Cell Biology.
  More results at FactBites »


 

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