DCPIP (2,6-dichlorophenol-indophenol) is a blue chemical compound used as a dye due to the fact that when it is in its oxidised state it is blue. Once reduced (by addition of electrons) it becomes colourless. This reaction can be reversed, and the colourless DCPIP will turn blue again, when it is oxidized. It is often used in measurements of the electron transport chain in plants as it has a higher affinity for electrons than ferridoxin. It is also commonly used to determine the concentration of ascorbic acid in a solution. A chemical compound is a chemical substance formed from two or more elements, with a fixed ratio determining the composition. ... Yarn drying after being dyed in the early American tradition, at Conner Prairie living history museum. ... Redox reactions include all chemical processes in which atoms have their oxidation number (oxidation state) changed. ... Redox reactions include all chemical processes in which atoms have their oxidation number (oxidation state) changed. ... In chemistry, concentration is the measure of how much of a given substance there is mixed with another substance. ... This article deals with the molecular aspects of ascorbic acid. ...
DCPIP is an oxidising agent and in its natural state is a blue solid. However if a solution of the dye (also blue) is titrated with a reducing agent (such as ascorbic acid) it gains electrons and the newly formed compound is colourless. In jewelry, a solid gold piece is the alternative to gold-filled or gold-plated jewelry. ... A reducing agent is the element or a compound in a redox (reduction-oxidation) reaction (see electrochemistry) that reduces another species. ... Properties The electron (also called negatron, commonly represented as e−) is a subatomic particle. ... A chemical compound is a chemical substance formed from two or more elements, with a fixed ratio determining the composition. ...
Uses
Determining the concentration of Ascorbic Acid
A solution of DCPIP is made by dissolving the solid in distilled water, the solution is then filtered to ensure accuracy. This solution is then titrated with the ascorbic acid solution until a faint pink colour persists for 15 seconds indicating the end point of the titration.
Reference
Derek Denby, Chemistry Review, May 1996
See also
Ascorbic Acid includes other methods for determining its concentration
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