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Encyclopedia > Electrochromatography

Electrochromatography is a chemical separation technique in analytical chemistry, biochemistry and molecular biology used to resolve and separate mostly large biomolecules such as proteins. It is a combination of size exclusion chromatography (gel filtration chromatography) and gel electrophoresis. These separation mechanisms operate essentially in superposition along the length of a gel filtration column to which an axial electric field gradient has been added. The molecules are separated by size due to the gel filtration mechanism and by electrophoretic mobility due to the gel electrophoresis mechanism. Additionally there are secondary chromatographic solute retention mechanisms.[1] [2] Analytical chemistry is the analysis of material samples to gain an understanding of their chemical composition and structure. ... Biochemistry is the study of the chemical processes and transformations in living organisms. ... Molecular biology is the study of biology at a molecular level. ... ... A representation of the 3D structure of myoglobin, showing coloured alpha helices. ... Equipment for running size exclusion chromatography. ... DNA electrophoresis apparatus. ...


See also

Pictured is a sophisticated gas chromatography system. ... Equipment for running size exclusion chromatography. ... DNA electrophoresis apparatus. ... Schematic representation of a protein electrophoresis gel In chemistry and medicine, protein electrophoresis is a method of analysing a mixture of proteins by means of gel electrophoresis, mainly in blood serum (blood plasma is not suitable). ... Electrofocusing, or isoelectric focusing, is a technique for separating different molecules by their electric charge differences (if they have any charge). ... Two-dimensional gel electrophoresis, commonly abbreviated as 2-DE or 2-D electrophoresis, is a form of gel electrophoresis commonly used to analyze proteins. ... Temperature gradient gel electrophoresis (TGGE) is a form of electrophoresis that studies the behavior of substances under different temperatures. ...

References

  1. ^ Analysis by Electromigration plus Chromatography Strain, H.H., Sullivan, .J. C., Anal.Chem. 23, 816 (1951)
  2. ^ Electrochromatography of Proteins , Basak, S., A. Velayudhan, K. Kohlmann and M. R. Ladisch, Journal of Chromatography A., 707, 69-76 (1995)

External links

  • Electrochromatography at Purdue University


 
 

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