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Nikon MicroscopyU: Color Balance in Digital Imaging (6862 words) |
 | Conceptually, coarse adjustments in white balance are required to bring the image sensor response into the appropriate range for the general illumination conditions (similar to choosing a category of film), while fine modifications are somewhat analogous to the use of color compensating filters in film-based photography. |
| Even when the illumination source and detector response are matched, light passing through the microscope is usually modified, somewhat unpredictably, by the specimen and any other components in the optical path. |
| In order to allow the white balance circuitry to evaluate the illumination at a brightness level similar to that existing with the specimen (and at approximately correct exposure settings), a neutral density filter may be inserted into the light path during the white balance adjustment, and then replaced with the specimen for actual imaging. |
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