Exon trapping is a molecular biology technique to identify potential exons in a fragment of eucaryoteDNA of unknown intron-exon structure. This is done to determine if the fragment is part of an expressed gene. Molecular biology is the study of biology at a molecular level. ... The exon portion of a DNA strand encodes a specific portion of a protein. ... Kingdoms Eukaryotes are organisms with complex cells, in which the genetic material is organized into membrane-bound nuclei. ... Space-filling model of a section of DNA molecule Deoxyribonucleic acid (DNA) or deoxyribose nucleic acid is a nucleic acid that contains the genetic instructions specifying the biological development of all cellular forms of life (and many viruses). ... Diagram of the location of introns and exons within a gene. ... The exon portion of a DNA strand encodes a specific portion of a protein. ... This stylistic schematic diagram shows a gene in relation to the double helix structure of DNA and to a chromosome (right). ...
The genomic fragment is inserted into the intron of a 'splicing vector' consisting of a known exon - intron - exon sequence of DNA. If the fragment does not contain exons (i.e., consists solely of intron DNA), it will be spliced out together with the vector's original intron. On the other hand, if exons are contained, they will be part of the mature mRNA after transcription (with all intron material removed). The presence of 'trapped exons' can be detected by an increase in size of the mRNA. Traditionally in medicine, a vector is an organism that does not cause disease itself but which spreads infection by conveying pathogens from one host to another. ... The interaction of mRNA in a eukaryote cell. ...
Exons are the regions of DNA within a gene that are not spliced out and which are retained in the final mRNA molecule.
In many genes, each exon codes for a specific portion of the complete protein, however, not all exons in a given gene will end up in the final protein due to the process of alternative splicing.
Exontrapping is a molecular biology technique that exploits the existence of the intron-exon architecture to find new genes.
In 1998, we described the structure of the RFC gene and the identification of alternative upstream non-coding exons, each transcribed from a separate promoter.
Most recently, a systematic study of these non-coding exons and promoters in assorted tissues identified an additional 4 non-coding exons and unique promoters, spanning more than 30 kb upstream from the translation start site.
It is trapped intracellularly after phosphorylation by thymidine kinase (TK), in a manner similar to the trapping of glucose analogs (FDG) after phosphorylation by hexokinase.
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