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Expression cloning is a technique in DNA cloning that uses expression vectors. Cloning is the process of creating an identical copy of an original. ... This article or section does not cite its references or sources. ...
Expression Vectors
Expression vectors are a specialized type of cloning vector in which the transcriptional and translational signals needed for the regulation of the gene of interest are included in the cloning vector. The transcriptional and translational signals may be synthetically created to make the expression of the gene of interest easier to regulate. Look up vector in Wiktionary, the free dictionary. ... The pGEX-3x plasmid is a popular cloning vector. ...
Purpose
Usually the ultimate aim of expression cloning is to produce large quantities of specific proteins. To this end, a bacterial expression clone may include a ribosomebinding site (Shine-Delgarno sequence) to enhance translation of the gene of interest's mRNA, a transcriptiontermination sequence, or, in eukaryotes, specific sequences to promote the post-translational modification of the protein product. A representation of the 3D structure of myoglobin, showing coloured alpha helices. ... Phyla Actinobacteria Aquificae Chlamydiae Bacteroidetes/Chlorobi Chloroflexi Chrysiogenetes Cyanobacteria Deferribacteres Deinococcus-Thermus Dictyoglomi Fibrobacteres/Acidobacteria Firmicutes Fusobacteria Gemmatimonadetes Lentisphaerae Nitrospirae Planctomycetes Proteobacteria Spirochaetes Thermodesulfobacteria Thermomicrobia Thermotogae Verrucomicrobia Bacteria (singular: bacterium) are unicellular microorganisms. ... As a word, clone was first coined by J.B.S. Haldane as subject for theoretical replication of a frog, though the term clone is derived from κλων, the Greek word for twig. In horticulture, the spelling clon was used until the twentieth century. ... Figure 1: Ribosome structure indicating small subunit (A) and large subunit (B). ... A binding site is a region on a protein to which specific ligands bind. ... In mathematics, a sequence is a list of objects (or events) arranged in a linear fashion, such that the order of the members is well defined and significant. ... Look up Transcription in Wiktionary, the free dictionary. ... Termination as a technical term has different meanings. ... Kingdoms Eukaryotes are organisms with complex cells, in which the genetic material is organized into membrane-bound nuclei. ... Posttranslational modification is the chemical modification of a protein after its translation. ...
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