Extractable Nuclear Antigens are soluble cytoplasmic and nuclear components that are antibody targets with over 100 differerent antigens discribed. The main 6 used in immunological laboratories for detection are Ro, La, Sm, RNP, Scl-70 and Jo1, which are screened for by Ouchterlony Double Immuno Diffusion techniques and confirmed by Immuno blotting Schematic of antibody binding to an antigen An antibody is a protein used by the immune system to identify and neutralize foreign objects like bacteria and viruses. ... An antigen is any molecule that is recognized by antibodies. ... Ro is an a priori constructed language created by Rev. ... Look up la on Wiktionary, the free dictionary. ... SM may stand for: Super Motard Master of Science, as designated in professional masters programs at MIT. Sadism and masochism, sometimes also written as S&M. Sailor Moon, a popular Japanese cartoon. ... Rede Nacional de Ensino e Pesquisa (RNP) (National Education and Research Network) is Brazils academic internet backbone. ...
Extractablenuclearantigen antibodies are a group of antibodies directed at nuclear, and in the case of Jo1, cytoplasmic antigens, which have particular associations with various connective tissue disorders.
Virtually all samples with positive ENA antibodies are ANA positive.
Antibodies to Jo-1 react primarily with cytoplasmic antigens (histidyl transfer RNA synthetase) and accordingly the ANA may be negative.
The Ro (SS-A) and La (SS-B) antigenic determinants appear to be related to one another because of the frequent coincidence of spontaneous anti-Ro and anti-La in the same autoimmune sera, and because of a tendency of the Ro and La immunoprecipitin lines in double immunodiffusion analysis to fuse.
A significant number of antigens from A, B, C, DR and DQ loci was investigated in 50 patients with primary Sj?gren's syndrome with positive autoantibodies against dsDNA, ssDNA, Ro (SS-A), La (SS-B), RNP antigens, as well as with higher titer of anticardiolipin antibodies from IgM and IgG classes.
The La (SS-B) autoimmune antigen is an RNA-binding protein that is present in both the nucleus and cytoplasm of eukaryotic cells, where it is found associated with RNA polymerase III transcripts.
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