Fast Protein Liquid Chromatography, usually referred to as FPLC, is a form of column chromatography used to separate or purify proteins from complex mixtures. It is very commonly used in biochemistry and enzymology. Columns used with an FPLC can separate macromolecules based on size, charge distribution, hydrophobicity, or biorecognition (as with affinity chromatography). Column chromatography in chemistry is the preparative application of chromatography. ... Biochemistry is the study of the chemical processes and transformations in living organisms. ... Ribbon diagram of the catalytically perfect enzyme TIM. An enzyme is a protein that catalyzes, or speeds up, a chemical reaction. ... Affinity chromatography is a biochemical separation method that combines size fractionation capability of gel permeation chromatography with the ability to design a stationary phase that reversibly binds to a known subset of molecules. ...
Trademark
FPLC is a trademark of GE Healthcare, formerly Pharmacia Biotech.
Typical columns
Typical columns used for protein purification include: Protein purification is the process of isolating proteins from a homogenate, which may comprise cell and tissue components, including DNA, cell membrane and other proteins. ...
Reversed phase or hydrophobic interaction — separates based on hydrophobicity which depends heavily on surface area
Affinity chromatography — columns which purify based on ligand affinity, such as a His-tagged protein would use a nickel column.
Ion-exchange chromatography, or the more general ion chromatography, is a process that allows the separation of ions and polar molecules based on the charge properties of the molecules. ... Gel filtration chromatography is a laboratory technique to separate biomolecules by size. ... Gel permeation chromatography (GPC) also known as size exclusion chromatography (SEC) is a chromatographic method in which molecules are separated based on their size. ... In chemistry, hydrophobic or lipophilic species, or hydrophobes, tend to be electrically neutral and nonpolar, and thus prefer other neutral and nonpolar solvents or molecular environments. ... Affinity chromatography is a biochemical separation method that combines size fractionation capability of gel permeation chromatography with the ability to design a stationary phase that reversibly binds to a known subset of molecules. ...
See also
Chromatography, an overview article covering all chromatographic techniques.
High performance liquid chromatography (HPLC) for column chromatography using high pressure.
Fast updating is said to be especially important for German patents because the period of opposition in which patent applications may be challenged is more limited than in some other countries.
It is said to provide an introduction to the science and technology of chromatography, as well as giving advanced references on the theory of the method.
Key references to recent literature and discussions on emerging technologies and applications in chromatography are also included, as are tables and figures illustrating and clarifying the text's technical points and applications.
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