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Untitled Document (375 words) |
 | We perform a proof-of-concept binding assay using enzymatic signal amplification in an array of femtoliter sized reaction vessels. |
| The current limit of detection (LOD) for binding streptavidin- b -galactosidase (S b G) to a biotinylated femtoliter array is 2.6 amoles (150 m L of 17 fM solution) using a target incubation time of 1 hour. |
| A portion of the fiber array for each S b G binding experiment containing (a) 128amol, (b) 51 amol, (c) 25 amol, (d) 7.5 amol, (e) 2.6 amol, and one of the two (f) controls, showing a representative number of active versus inactive reaction chambers. |
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