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Encyclopedia > Gene sequencing

Gene sequencing refers to the process of recording the exact sequence of nucleotides in the section of an organism's DNA corresponding to a specific gene. The complete genetic sequences of humans and many other organisms have been determined. Researchers sometimes sequence specific genes of an individual with a certain phenotype (such as a disease), in an attempt to discover the phenotype's genetic basis.


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Sequencing of DNA has been one of the major feats in molecular biology.
Two independent sequencing methods were invented in the middle seventies, one by Walter Gilbert, called the Maxam-Gilbert method or chemical method, and a second one by Fred Sanger called Sanger or chain termination method.
The sequencing reaction has been modified into a PCR reaction, and the old way using radioactively labelled deoxynucleotides has been improved using dye-labelled bases.
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