Glostrup is a municipality in eastern Denmark, in the county of Copenhagen on the island of Zealand. The municipality covers an area of 13 km², and has a total population of 20,229. A municipality or general-purpose district (compare with: special-purpose district) is an administrative local area generally composed of a clearly defined territory and commonly referring to a city, town, or village government. ... Københavns Amt (English: Copenhagen County) is a county in eastern Denmark, located on the island of Zealand, covering the municipalities in metropolitan Copenhagen except Copenhagen and Frederiksberg. ... This article is about the Danish island. ... Square kilometre (US spelling: Square kilometer), symbol km², is an SI unit of surface area. ...
Immune effector function analyses with the Lewis Y antigen system utilised the humanised anti-Lewis Y mAb hu3S193 (35) and IgG1 class-matched control huA33 (36) antibodies produced by the Biological Production Facility (Ludwig Institute for Cancer Research, Melbourne, Australia).
Anti-CD3-FITC/CD4-RPE, anti-CD3-FITC/CD8-RPE, anti-CD19-FITC, anti-CD20-RPE, anti-CD14-RPE, anti-CD15-FITC, mouse IgG1-FITC/IgG1-RPE negative control, mouse IgM-FITC negative control, and mouse IgG2a-RPE negative control were purchased from DAKO (Glostrup, Denmark).
Antibody staining was detected using a secondary biotinylated antibody (LSAB Kit; Dako, Glostrup, Denmark or Silenus, Melbourne, Australia) followed by incubation with horse radish peroxidase conjugated alpha-biotin (LSAB Kit; Dako, Glostrup, Denmark or Vectastain ABC Elite Kit, Vector Laboratories, Burlingame, CA, USA) and visualized by immersing in chromogen substrate AEC (Sigma-Aldrich, St. Louis, MO, USA).
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