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Two novel assays for the detection of haemin-binding properties of antimalarials evaluated with compounds isolated ... (4705 words) |
 | the inhibition of the reaction(s) of GSH with haemin; |
| on the reversal of the inhibition by haemin of the dopachrome |
| the reversal of haemin inhibition of MIF dopachrome |
| Inter Research » DAO » v48 » n2 » p109-115 (362 words) |
| Proteinase K treatment of whole cells markedly reduced the binding of haemin, indicating that protein receptors located at the cell surface are involved in the binding. |
| This was confirmed by the observation that isolated total as well as outer membrane proteins from all the strains, regardless of the iron levels of the media, were able to bind haemin, with the outer membranes showing the strongest binding. |
| Haemin binding by membrane protein extracts was not affected by heat treatment but was almost completely abolished by Proteinase K treatment, suggesting the presence of thermostable protein receptors for haemin. |
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