Transmission image of HeLa cells, with overlay of Hoechst 33258 staining (blue). The leftmost cell is in the anaphase stage of mitosis; its chromosomes fluoresce brightly because they contain highly concentrated DNA.
The Hoechst stains are part of a family of fluorescentstains for labelling DNA in fluorescence microscopy. Two of these closely related bis-benzimides are commonly used: Hoechst 33258 and Hoechst 33342. HeLa cells dividing under electron microscopy Hela is also the German name for Hel, Poland and the cruiser SMS Hela In biological and medical research, a HeLa cell is a cell which is derived from cervical cancer cells taken from a woman named Henrietta Lacks, who died from the cancer... A lung cell of a newt during early anaphase of mitosis. ... Light micrograph of a newt lung cell in early anaphase of mitosis. ... Figure 1: Chromosome. ... Fluorescence induced by exposure to ultraviolet light in vials containing various sized cadmium selenide (CdSe) quantum dots. ... For staining in the sense of wood staining, see Wood staining. ... Space-filling model of a section of DNA molecule Deoxyribonucleic acid (DNA) is a nucleic acid that contains the genetic instructions specifying the biological development of all cellular forms of life (and many viruses). ... Microscopy is any technique for producing visible images of structures or details too small to otherwise be seen by the human eye, using a microscope or other magnification tool. ...
Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescence light around an emission maximum at 461 nm. The Hoechst stains may be used on live or fixed cells, and are often used as a substitute for another nucleic acid stain, DAPI. Ultraviolet (UV) radiation is electromagnetic radiation of a wavelength shorter than that of the visible region, but longer than that of soft X-rays. ... A nanometre (American spelling: nanometer) is 1. ... Structural formula of DAPI DAPI or 4,6-diamidino-2-phenylindole is a fluorescent stain that binds strongly to DNA. It is used extensively in fluorescence microscopy. ...
Because the Hoechst stains bind to DNA, they can disrupt DNA replication during cell division. Consequently they are potentially mutagenic and carcinogenic. Care should be taken in their handling and disposal. DNA replication - Wikipedia /**/ @import /w/skins-1. ... Light micrograph of a newt lung cell in early anaphase of mitosis. ... In biology, a mutagen (Latin, literally origin of change) is an agent that changes the genetic information (usually DNA) of an organism and thus increases the number of mutations above the natural background level. ... In pathology, a carcinogen is any substance or agent that promotes cancer. ...
Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes.
Stains may be used to define and examine bulk tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells, for instance), or organelles within individual cells.
Gram staining uses crystal violet to stain cell walls, iodine as a mordant, and a fuchsin or safranin counterstain to mark all bacteria.
The Hoechst 33342 dye is commonly used in combination with labeling by 5-bromo-2'-deoxyuridine (BrdU, B23151) to distinguish the compact chromatin of apoptotic nuclei, to identify replicating cells and to sort cells based on their DNA content (Section 15.4, Section 15.5).
Hoechst 33342 is commonly used to distinguish the compact chromatin of apoptotic nuclei, in combination with BrdU labeling to identify replicating cells and to sort cells based on DNA content (Section 15.5).
Staining with the SYTO 11 dye was used to follow the movement of cells during development in whole-mount zebrafish embryos.
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