The Hope Valley Line is a railway line in England linking Sheffield with Manchester. It was completed in 1894.

From Sheffield station, trains head down the Midland Main Line to Dore, where the Hope Valley Line branches off to run through the Totley Tunnel. It emerges in Derbyshire, where it passes through stunning scenery and the railway stations of Grindleford, Hathersage, Bamford, Hope and Edale. It then enters the Cowburn Tunnel and reemerges two miles west.

The line then runs through Chinley before splitting, the northern branch running via New Mills toward Manchester Piccadilly, the southern passes through the Disley Tunnel before merging with the Buxton line and then heading to Stockport.

Passenger services on the line are currently operated by Northern Rail (previously First North Western) and TransPennine Express.