Hybridisation is the process of combining complementary, single-stranded nucleic acids into a single molecule. Nucleotides will bind to their complement under normal conditions, so two perfectly complementary strands will bind to each other readily. Conversely, due to the different geometries of the nucleotides, a single inconsistency between the two strands will prevent them from binding. The process can be reversed by heating the molecule. A nucleic acid is a complex, high-molecular-weight biochemical macromolecule composed of nucleotide chains that convey genetic information. ... In science, a molecule is the smallest particle of a pure chemical substance that still retains its chemical composition and properties. ... A nucleotide is an organic molecule consisting of a heterocyclic nucleobase (a purine or a pyrimidine), a pentose sugar (deoxyribose in DNA or ribose in RNA), and a phosphate or polyphosphate group. ...
Molecular techniques such as FISH and Southern blots use hybridization probes to identify RNA. Fluorescent in situ hybridization (FISH) is a process which vividly paints chromosomes or portions of chromosomes with fluorescent molecules. ... A Southern blot is a method in molecular biology of enhancing the result of an agarose gel electrophoresis by marking specific DNA sequences. ... A hybridization probe is a short piece of DNA (on the order of 100-500 bases) that is denatured (by heating) into single strands and then radioactively labelled, usually with phosphorus (32P or 33P). ... Ribonucleic acid (RNA) is a nucleic acid consisting of a string of covalently-bound nucleotides. ...
References
"Glossary of Biotechonogy Terms (http://www.cs.wustl.edu/~jbuhler/research/array/glossary.html#hybridize)." from "Anatomy of a Comparative Gene Expression Study" by J Buhler. Accessed on May 7, 2005. May 7 is the 127th day of the year in the Gregorian Calendar (128th in leap years). ... 2005 is a common year starting on Saturday of the Gregorian calendar and is the current year. ...
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