Integrase is a protein produced by a virus that enables genetic material that is helpful to the virus, proviral DNA, to be integrated into the DNA of the infected cell.
HIV integrase is a 32 kDaprotein produced from the C-terminal portion of the Pol gene product. The integrase protein contains three domains: an N-terminal HH-CC zinc finger domain believed to be partially responsible for multimerization, a central catalytic domain and an C-terminal DNA binding domain. Integrase is responsible for the integration of proviral DNA into the host genome, which is essential for HIV replication. Integrase, therefore, remains a promising potential target for new anti-HIV therapeutics. HIV (Human Immunodeficiency Virus) is a retrovirus that infects cells of the human immune system. ... The atomic mass unit (amu), unified atomic mass unit (u), or dalton (Da), is a small unit of mass used to express atomic masses and molecular masses. ... A representation of the 3D structure of myoglobin, showing coloured alpha helices. ... A zinc finger is part of a protein that can bind to DNA. Zinc finger domains typically consist of two β sheets, each carrying a cysteine residue, and an α helix carrying two histidine residues. ... Space-filling model of a section of DNA molecule Deoxyribonucleic acid (DNA) or deoxyribose nucleic acid is a nucleic acid that contains the genetic instructions specifying the biological development of all cellular forms of life (and many viruses). ...
See also: retrovirus Genera Alpharetrovirus Betaretrovirus Gammaretrovirus Deltaretrovirus Epsilonretrovirus Lentivirus Spumavirus A retrovirus is a virus which has a genome consisting of two plus sense RNA molecules, which may or may not be identical. ...
Integrase is a protein produced by a virus that enables genetic material that is helpful to the virus, proviral DNA, to be integrated into the DNA of the infected cell.
The integraseprotein contains three domains: an N-terminal HH-CC zinc finger domain believed to be partially responsible for multimerization, a central catalytic domain and an C-terminal DNA binding domain.
Integrase is responsible for the integration of proviral DNA into the host genome, which is essential for HIV replication.
Integrase functions in an as-yet biochemically undefined complex of viral and cellular proteins referred to as the preintegration complex (Farnet and Haseltine, 1991 and Brown et al., 1987).
Integrase was added to 20 ul of resin in a 4:1 molar ratio of integrase to DNA (1.92 nmol per sample) and incubated for 30 min.
Integrase is a 288 amino acid protein with a codon usage that is skewed against high-level expression in E. coli.
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