Joan, Infanta of Spain (Spanish: 'Juana'), of the Habsburg family, was the daughter of Emperor Charles V who was the first king of united Spain (officially King of Aragon and king of Castile), and his consort Infanta Isabel of Portugal, daughter of King Manuel I of Portugal. Habsburg (sometimes spelled Hapsburg, but never so in official use) was one of the major ruling houses of Europe. ... Charles V Holy Roman Emperor and King of Spain Charles V (Spanish: Carlos I, Dutch: Karel V, German: Karl V.) (24 February 1500–21 September 1558) was effectively (the first) King of Spain from 1516 to 1556 (in principle, he was from 1516 king of Aragon and from 1516 guardian... Isabella of Portugal (1503 - 1539) was princess of Portugal. ... Manuel I of Portugal (Archaic Portuguese: Manoel I, English: Emanuel I), the Fortunate (Port. ...

As such, Juana was an Archduchess of Austria, Infanta of Castile and of Aragon, Princess of Burgundy and Flanders, daughter of Roman Emperor, etc.

She married her first cousin, Infante João de Portugal, who was the heir of Portugal, the sole surviving son of her paternal aunt Catherine of Habsburg and her maternal uncle King John III of Portugal. Their teenage marriage led to pregnancy (Joao was 15 y.o. when his wife conceived), and their only child Sebastian of Portugal was born posthumously in 1554 a couple of weeks after the teenager father Joao had perished of tuberculosis at the age of 16 years. Prince João of Portugal (June 3, 1537 - January 2, 1554) (in English: John) was the eighth son of King John III of Portugal by his wife Catherine of Habsburg, (princess of Spain) daughter of Philip of Flanders and Joanna of Castile. ... Catherine of Austria, of the Habsburg family, princess of Spain, born a daughter of Philip, Archduke, Lord of Flanders etc and Joanna of Castile, as such Archduchess of Austria, Infanta of Castile, Princess of Burgundy and Flanders, sister of King of Aragon and Roman Emperor, etc. ... This article needs copyediting (checking for proper English spelling, grammar, usage, etc. ... Sebastian of Portugal Sebastião I (English: Sebastian) the Desired (Port. ... Events February 12 - After claiming the throne of England the previous year, Lady Jane Grey is beheaded for treason alongside her husband. ... Tuberculous lungs show up on an X-ray image Tuberculosis is an infection with the bacterium Mycobacterium tuberculosis, which most commonly affects the lungs (pulmonary TB) but can also affect the central nervous system (meningitis), lymphatic system, circulatory system (miliary TB), genitourinary system, bones and joints. ...