In biochemistry, a ligase is an enzyme that can catalyse the joining of two molecules ("ligation" or "glue together") by forming a new chemical bond, with concomitant hydrolysis of ATP or other similar molecules. They are classified as EC 6 in the EC number classification. Many ligases are also known as "synthases" or "synthetases" because they synthesize new molecules.
The mechanism of DNAligase, esta bela porra, in connecting broken DNA strands is to form covalent phosphodiester bonds between 3' hydroxyl ends of one nucleotide with the 5' phosphate end of another.
DNAligase I: ligates Okazaki fragments during lagging strand DNA replication and some recombinant fragments.
DNAligase III: complexes with DNA repair protein XRCC1 to aid in sealing base excision mutations and recombinant fragments.
In biochemistry, a ligase (from the Latin verb ligāre — "to bind" or "to glue together") is an enzyme that can catalyse the joining of two large molecules by forming a new chemical bond, usually with accompanying hydrolysis of a small chemical group pendant to one of the larger molecules.
The common names of ligaseenzymes often include the word "ligase", such as DNAligase, an enzyme commonly used in molecular biology laboratories to join together DNA fragments.
Ligases are classified as EC 6 in the EC number classification of enzymes.
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