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Encyclopedia > Lowry protein assay

Principle


Under alkaline conditions the divalent copper ion forms a complex with peptide bonds in which it is reduced to a monovalent ion. Monovalent copper ion and the radical groups of tyrosine, tryptophan, and cysteine react with Folin reagent to produce an unstable product that becomes reduced to molybdenum/tungsten blue.


  Results from FactBites:
 
Protein Assay (324 words)
Protein assays determine the amount of protein in an unknown solution.
Protein assays enable biochemists to characterize one of the two defining properties of enzymes (the other being activity).
Proteins are polymers of amino acids, where amino acid units are joined by peptide bonds which have partial double bond character and absorb light.
Assay (296 words)
An assay is a procedure where the concentration of a component part of a mixture is determined.
There are numerous applications of an assay, such as an antigen capture assay, bioassay, competitive protein binding assay, four-point assay, immunoassay, microbiological assay, stem cell assay, and many others.
Assays are regularly utilized in scientific research laboratories.
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