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Cell culture medium - Patent 4038139 (1647 words) |
 | In a method for culturing lymphoblast cells of the type involving culturing cells in an aqueous nutrient medium containing animal serum the improvement which comprises using swine serum in combination with a surfactant to inhibit the precipitation of serum protein. |
| Human lymphoblast cells may be grown through successive subcultures using a conventional cell culture medium in which the serum component consists of swine serum. |
| MICH strain human lymphoblasts cells age grown in shake flask cultures in conventional media to which are added 10% foetal calf serum, swine, lamb, chicken, newborn calf or horse serum, each of these sera being commerical preparations. |
| Signal transduction of somatostatin in human B lymphoblasts -- Rosskopf et al. 284 (1): C179 -- AJP - Cell Physiology (6211 words) |
| Lymphoblast RNA was prepared and reverse transcribed using Superscript reverse transcriptase as described (44). |
| B lymphoblasts were stimulated for 2 min with 100 nM SST, 100 nM platelet-activating factor (PAF), or for 15 min with 250 nM of the phorbol ester TPA or remained nonstimulated (control). |
| Inhibition by somatostatin of the proliferation of T-lymphocytes and Molt-4 lymphoblasts. |
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