Nocodazole is an anti-neoplasticagent which exerts its effect by depolymerizingmicrotubules. Microtubule formation is an important structural feature of cells as they enter mitosis. Several drugs including vincristine and colcemid, are similar to nocodazole in that they interfere with microtubules and cause arrest in G2/M phase of the cell cycle. As nocodazole affects the cellular cytoskeleton, it is often used in cell biology experiments as a control: for example, some dominant negative Rhosmall GTPases cause a similar effect as nocodazole, and constitutively activated mutants often reverse or negate the effect. Also, nocodazole is frequently used in cell biology laboratories to synchronize the cell division cycle. Cells treated with nocodazole enter mitosis but cannot form metaphasespindles because microtubules (of which the spindles are made) cannot polymerise. This activates the spindle assembly checkpoint, and the cells arrest in prometaphase. For cell synchronization experiments, nocodazole is usually used at a concentration of 100ng/mL of culture medium for a duration of 12-18 hrs. ... A chemical compound is a chemical substance consisting of two or more different chemically bonded chemical elements, with a fixed ratio determining the composition. ... Polymer is a term used to describe large molecules consisting of repeating structural units, or monomers, connected by covalent chemical bonds. ... Microtubules are protein structures found within cells, one of the components of the cytoskeleton. ... Vincristine (Oncovin®) is an alkaloid from the Madagascar periwinkle (Catharanthus roseus, formerly Vinca rosea and hence its name). ... Cell biology (also called cellular biology or cytology, from the Greek kytos, container) is an academic discipline that studies cells. ... 1. ... In biology, small GTPases are small (20-25 KDa) proteins that bind to guanosine triphosphate GTP. This family of proteins are homologous to Ras GTPases and also called the Ras superfamily GTPases. ... Mitosis divides genetic information during cell division. ... An image of a newt lung cell stained with flourescent dyes during metaphase. ... The mitotic spindle is a structure of the eukaryotic cytoskeleton involved in mitosis and meiosis. ... The spindle checkpoint blocks entry into anaphase until all chromosomes are properly attached to the mitotic spindle. ... Yeah. ...
My research has centered on the experimental investigation of regulatory mechanisms in ovarian function, especially on the control and cellular mechanism of oocyte meiosis using amphibian, insect and teleost ovaries as experimental model systems.
The research has focused on the effect of steroids and cytoskeleton-active agents such as taxol, nocodazole, soluble acrylamide and colcemid on the processes of nuclear migration within the oocyte and dissolution of the nuclear envelope during meiotic cell division (prophase I - metaphase I transition).
This work employed molecular approaches including the use of monoclonal antibody probes for cytoskeletal proteins, Western blotting, confocal immunofluorescence microscopy, and morphological analysis at the ultrastructural level using immunogold procedures with transmission EM.
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