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Encyclopedia > Numerical aperture

Numerical aperture is a technical term of multiple uses:


  Results from FactBites:
 
Nikon MicroscopyU: Common Formulas in Microscopy: Numerical Aperture (459 words)
In the numerical aperture equation, n represents the refractive index of the medium between the objective front lens and the specimen, and
The numerical aperture of a microscope objective is a measure of its ability to gather light and resolve fine specimen detail at a fixed object distance.
The numerical aperture of an objective is also dependent, to a certain degree, upon the amount of correction for optical aberration.
Olympus Microscopy Resource Center: Anatomy of the Microscope - Numerical Aperture and Resolution (2208 words)
Numerical aperture determines the resolving power of an objective, but the total resolution of a microscope system is also dependent upon the numerical aperture of the substage condenser.
As the numerical aperture and light cone angle of an objective increases however, the size of the Airy disk decreases as illustrated in Figure 4(b) and Figure 4(c).
Careful positioning of the substage condenser aperture diaphragm is also critical to the control of numerical aperture and indiscriminate use of this diaphragm can lead to image degradation (as discussed in the section on substage condensers).
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