Ponceau S

Ponceau S, Acid Red 112, or C.I. 27195 is a sodium salt of a diazo dye that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or PVDF membranes (Western blotting), as well as on cellulose acetate membranes. A Ponceau S stain is easily reversed with water washes, facilitating subsequent immunological detection. Common stain formulations include 0.1% (w/v) Ponceau S in 5% acetic acid or 2% (w/v) Ponceau S in 30% TCA and 30% sulfosalicylic acid. Image File history File links No higher resolution available. ... Image File history File links No higher resolution available. ... In chemistry, azo compounds generally have a molecular formula of the form R-N=N-R, in which R and R can be either aromatic or aliphatic. ... Staining is a biochemical technique of adding a class-specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. ... A representation of the 3D structure of myoglobin, showing coloured alpha helices. ... Skeletal formula of nitrocellulose Ball-and-stick model of a section of nitrocellulose Nitrocellulose (also: cellulose nitrate, flash paper) is a highly flammable compound formed by nitrating cellulose through, for example, exposure to nitric acid or another powerful nitrating agent. ... PVDF, or PolyVinylidine DiFluoride, is a highly non-reactive and pure thermoplastic fluoropolymer. ... Figure 1. ... Cellulose acetate, first prepared in 1865, is the acetate ester of cellulose. ... Immunology is a broad branch of biomedical science that covers the study of all aspects of the immune system in all organisms. ... Acetic acid, also known as ethanoic acid, is an organic chemical compound best recognized for giving vinegar its sour taste and pungent smell. ... The term TCA can refer to more than one thing: TCA is the tricarboxylic acid cycle or citric acid cycle (Krebs Cycle) TCA is Trans Canada Airlines TCA is an acronym for tricyclic antidepressants TCA is shorthand for 2,4,6-trichloroanisole, the chief cause of cork taint in wine. ... Sulfosalicylic acid is used in urine tests to determine urine protein content. ...

Its CAS number is [6226-79-5] and its SMILES structure is OC1=C(N=NC3=C C=C(N=NC4=CC=C (S(=O)(O)=O)C=C4) C=C3S(=O)(O)=O) C2=C(C=C(S(=O) (O)=O)C=C2)C=C1 S(=O)(O)=O. CAS registry numbers are unique numerical identifiers for chemical compounds, polymers, biological sequences, mixtures and alloys. ... The simplified molecular input line entry specification or SMILES is a specification for unambiguously describing the structure of chemical molecules using short ASCII strings. ...

See also

• Western blotting
• Coomassie
• Ponceau family of Azo dyes