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Quorum sensing is the process by which many bacteria coordinate gene expression according to the local density of bacteria producing signaling molecules. Image File history File links This is a lossless scalable vector image. ... Phyla Actinobacteria Aquificae Chlamydiae Bacteroidetes/Chlorobi Chloroflexi Chrysiogenetes Cyanobacteria Deferribacteres Deinococcus-Thermus Dictyoglomi Fibrobacteres/Acidobacteria Firmicutes Fusobacteria Gemmatimonadetes Lentisphaerae Nitrospirae Planctomycetes Proteobacteria Spirochaetes Thermodesulfobacteria Thermomicrobia Thermotogae Verrucomicrobia Bacteria (singular: bacterium) are unicellular microorganisms. ... Gene expression, or simply expression, is the process by which the inheritable information which comprises a gene, such as the DNA sequence, is made manifest as a physical and biologically functional gene product, such as protein or RNA. Several steps in the gene expression process may be modulated, including the... Nitric oxide is a quite salient signaling molecule, instrumental in both learning and memory. ...

Consequences

The consequence of quorum sensing is the coordination of certain behavior or actions between bacteria, based on the local density of bacteria. Quorum sensing can occur within a single bacterial species as well as between disparate species, and can regulate a host of different processes, essentially serving as a simple communication network.

For example, opportunistic bacteria, such as Pseudomonas aeruginosa can grow within a host without harming it, until they reach a certain concentration. Then they become aggressive, their numbers sufficient to overcome the host's immune system and form a biofilm, leading to disease. It is hoped that the therapeutic enzymatic degradation of the signalling molecules will prevent the formation of such biofilms and possibly weaken established biofilms. Disrupting the signalling process in this way is called quorum quenching. Binomial name Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 Synonyms Bacterium aeruginosum Schroeter 1872 Bacterium aeruginosum Cohn 1872 Micrococcus pyocyaneus Zopf 1884 Bacillus aeruginosus (Schroeter 1872) Trevisan 1885 Bacillus pyocyaneus (Zopf 1884) Flügge 1886 Pseudomonas pyocyanea (Zopf 1884) Migula 1895 Bacterium pyocyaneum (Zopf 1884) Lehmann and Neumann 1896 Pseudomonas polycolor... A scanning electron microscope image of a single neutrophil (yellow), engulfing anthrax bacteria (orange). ... Staphylococcus aureus biofilm on an indwelling catheter. ... This article is about the medical term. ...

Modelling

The relevant mathematics for the behavior and coordination of the bacteria is found in the allometric scaling equation known as Kleiber's Law, relating metabolic rate to organism mass and metabolic efficiency [P = W^(4μ-1)/4μ] where P is metabolic rate, W is organism mass, and μ is metabolic efficiency - the ratio of rate of reduction reactions to rate of flow of energy from oxidation reactions. When the numbers are run for this equation for values of μ from 0 to 100%, and for values of W from e-13 grams to e+13 grams, what is revealed is that extremely small organisms, the size of bacteria, have very high metabolic rates which drop off rapidly with increases in mass. The equation implies then that cellular division of bacteria is a response to this diminution, an attempt to increase metabolic rate of the individual bacterium through its loss of mass by division. Notice that quorum sensing is dependent upon the number or mass density of bacteria. The equation is relevant on this score.

Unless the proliferating bacteria are diffused by circumstance, they remain in close proximity to each other. As the bacterial mass increases what is seen is a colony of bacteria, a new organism of sorts, still very, very small. The metabolic rate of the colony includes, but is not limited to, the basal metabolic rates of all its constituent bacteria, and is subject to the same loss of metabolic rate that comes with mass increase for the individual bacterium. These colonies can at times grow to considerable size, and are responsible for stromatolites, the oldest fossils on earth found in the coastal waters off Australia. Pre-Cambrian stromatolites in the Siyeh Formation, Glacier National Park. ...

But the mathematics also shows that with increases in mass, loss of metabolic rate is not so severe if μ or metabolic efficiency also increases. The math shows that increases in mass result in loss of metabolic rate for all organisms with a μ of less than 25%, and that as they approach this value for μ, the loss of metabolic rate becomes less with further increases in mass. Furthermore, the math shows that for things as small as single-celled organisms [e-5 grams], at μ greater than 25%, metabolic rate rapidly approaches zero unless mass is increased, not decreased. Consideration of the term μ reveals that to increase the value for μ either available energy must be reduced [the denominator, rate of flow of energy from oxidative sources], or the rate of reduction reactions [the numerator] must be increased.

Diminishing the denominator through the restriction of energy is something that usually takes place from circumstance, energy not being free. Increasing the numerator is done by the bacteria through energy expenditure appearing as the chemical production of organic substances like biofilms, or the generation of luminescence. In other words, at very small mass, biological organisms like bacteria and bacterial colonies, preserve their metabolic rates and perpetuate their existence and growth, through either division, the production of organic molecules, or the generation of light; and that at over 25% μ, to keep metabolic rate from crashing, very small things must increase in mass, something that follows from increased energy expenditure [the numerator of μ] in the creation of organic molecules.

What the math makes clear is that the purpose of quorum sensing is not a conscious attempt on the part of bacteria to coordinate behavior. The purpose of quorum sensing seems to be a chemical response of very small biological organisms to recoup loss of metabolic rate that follows from increases in mass at a value for μ below 25%. Attempts to understand the purpose of quorum sensing solely in terms of the chemical details of the mechanics of receptors and inducers, cloud understanding of the purpose of quorum sensing, and rest ultimately on a bit of quasi-anthropomorphism.

Methods and mechanisms

Bacteria that use quorum sensing produce and secrete certain signaling compounds (called autoinducers or pheromones), one example of which are N-acyl homoserine lactones (AHL). These bacteria also have a receptor that can specifically detect the AHL (inducer). When the inducer binds the receptor, it activates transcription of certain genes, including those for inducer synthesis. There is a low likelihood of a bacterium detecting its own secreted AHL. Look up chemical compound in Wiktionary, the free dictionary. ... Fanning honeybee exposes Nasonov gland (white-at tip of abdomen) releasing pheromone to entice swarm into an empty hive A pheromone is a chemical that triggers an innate behavioural response in another member of the same species. ... Homoserine Lactone is a signaling chemical involved in microbiological quorum sensing. ... In biochemistry, a receptor is a protein on the cell membrane or within the cytoplasm or cell nucleus that binds to a specific molecule (a ligand), such as a neurotransmitter, hormone, or other substance, and initiates the cellular response to the ligand. ... An inducer is a molecule in biology that starts gene expression. ... A micrograph of ongoing gene transcription of ribosomal RNA illustrating the growing primary transcripts. ... For other uses, see Gene (disambiguation). ...

When only a few other bacteria of the same kind are in the vicinity, diffusion reduces the concentration of the inducer in the surrounding medium to almost zero, so the bacteria produce little inducer. With many bacteria of the same kind, the concentration of the inducer passes a threshold, whereupon more inducer is synthesised. This forms a positive feedback loop, and the receptor becomes fully activated. This induces the up regulation of other specific genes, such as luciferase in V. fischeri. This is useful since a single V. fischeri bacterium that is luminescent would have no evolutionary advantage and would be wasting energy. diffusion (disambiguation). ... Positive feedback is a feedback system in which the system responds to the perturbation in the same direction as the perturbation (It is sometimes referred to as cumulative causation). ... Luciferase is a generic name for enzymes commonly used in nature for bioluminescence. ...

In Escherichia coli, AI-2 is produced by the lsr operon, encoding an ABC transporter which imports AI-2 into the cells during the early stationary (latent) phase of growth. AI-2 is then phosphorylated by lsrK and the newly produced phospho-AI-2 can either be internalized or used to suppress lsrR, an inhibitor of the lsr operon (thereby activating the operon). The lsr operon is also thought to be inhibited by dihydroxyacetone phosphate (DHAP) through its competitive binding to lsrR. Glyceraldehyde 3-phosphate has also been shown to inhibit the lsr operon through cAMP-CAPK-mediated inhibition. This explains why when grown with glucose E. coli will lose the ability to internalize AI-2 (because of catabolite repression). When grown normally, AI-2 presence is transient. E. coli redirects here. ... An operon is a group of key nucleotide sequences including an operator, a common promoter, and one or more structural genes that are controlled as a unit to produce messenger RNA (mRNA). ... ATP-binding cassette transporter genes (ABC-transporter genes) are a superfamily of genes which encode the ABC-transporter proteins. ... DHAP (or Dihydroxyacetonephosphate) is a biochemical compound involved in many reactions, from the Calvin Cycle in plants to the ether-lipid biosynthesis process in Leishmania mexicana. ... G3P (structure) Glyceraldehyde 3-phosphate (G3P) is an intermediate in both glycolysis and gluconeogenesis. ...

A first X-ray structure of a receptor (LuxP) was discovered in Vibrio harveyi in 2002, together with its inducer (AI-2), which is one of the few biomolecules containing boron.^[1] Autoinducer-2 is conserved among many bacterial species, including Escherichia coli, an enteric bacterium and model organism for Gram negative bacteria. Autoinducer-2 appears to be used for interspecies communication because of this conservation. X-ray crystallography is a technique in crystallography in which the pattern produced by the diffraction of x-rays through the closely spaced lattice of atoms in a crystal is recorded and then analyzed to reveal the nature of that lattice. ... Vibrio harveyi is a species of marine bioluminescent vibrio species almost ecologically identical to Vibrio fischeri. ... A representation of the 3D structure of myoglobin, showing coloured alpha helices. ... General Name, Symbol, Number boron, B, 5 Chemical series metalloids Group, Period, Block 13, 2, p Appearance black/brown Standard atomic weight 10. ...

Role in specific organisms

Quorum sensing was first observed in Vibrio fischeri, a bioluminiscent bacterium that lives as a symbiont in the light-producing organ of the Hawaiian bobtail squid. When V. fischeri cells are free-living (or planktonic), the autoinducer is at low concentration and thus cells do not luminesce. In the light organ of the squid (photophore), they are highly concentrated (about 10¹¹ cells/ml) and transcription of luciferase is induced, leading to bioluminescence. Binomial name Vibrio fischeri (Beijerinck 1889) Lehmann & Neumann 1896 Vibrio fischeri is a rod-shaped bacterium found globally in the marine environments. ... For other uses, see Symbiosis (disambiguation). ... Families Idiosepiidae Sepiolidae Bobtail squid are a group of cephalopods closely related to cuttlefish. ... A photophore is a light-emitting organ which appears as luminous spots on various marine fishes. ... Luciferase is a generic name for enzymes commonly used in nature for bioluminescence. ... Bioluminescence is the production and emission of light by a living organism as the result of a chemical reaction during which chemical energy is converted to light energy. ...

Processes possibly regulated or partially regulated by AI-2-mediated quorum sensing in E. coli include cell division. In other species such as Pseudomonas aeruginosa quorum-related processes include biofilm development, exopolysaccharide production, and cell aggregation. AI-2 was found to increase expression of sdiA, a transcriptional regulator of promoters which promote ftsQ, part of the ftsQAZ operon essential for cell division. See also Entamoeba coli. ... This does not adequately cite its references or sources. ... Binomial name Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 Synonyms Bacterium aeruginosum Schroeter 1872 Bacterium aeruginosum Cohn 1872 Micrococcus pyocyaneus Zopf 1884 Bacillus aeruginosus (Schroeter 1872) Trevisan 1885 Bacillus pyocyaneus (Zopf 1884) Flügge 1886 Pseudomonas pyocyanea (Zopf 1884) Migula 1895 Bacterium pyocyaneum (Zopf 1884) Lehmann and Neumann 1896 Pseudomonas polycolor... Staphylococcus aureus biofilm on an indwelling catheter. ... Polysaccharides (sometimes called glycans) are relatively complex carbohydrates. ...

Streptococcus pneumoniae uses quorum sensing to become competent. Binomial name (Klein 1884) Chester 1901 Streptococcus pneumoniae, or pneumococcus, is a Gram-positive, alpha-hemolytic diplococcus bacterium and a member of the genus Streptococcus. ... Competent cells are bacteria which can accept extra-chromosomal DNA. Cells can be made competent in several ways. ...

Quorum Sensing in Pseudomonas aeruginosa

Regulation of gene expression can occur through cell-cell communication or quorum sensing (QS) via the production of small molecules called autoinducers. QS is known to control expression of a number of virulence factors. Another form of gene regulation which allows the bacteria to rapidly adapt to surrounding changes is through environmental signaling. Recent studies have discovered that anaerobiosis can significantly impact the major regulatory circuit of QS. This important link between QS and anaerobiosis has a significant impact on production of virulence factors of this organism.^[2] Gene expression, or simply expression, is the process by which the inheritable information which comprises a gene, such as the DNA sequence, is made manifest as a physical and biologically functional gene product, such as protein or RNA. Several steps in the gene expression process may be modulated, including the... Virulence refers to the degree of pathogenicity of a microbe, or in other words the relative ability of a microbe to cause disease. ... Gene regulation is the general term for cellular control of protein synthesis at the DNA-RNA transcription step. ... Phyla Actinobacteria Aquificae Chlamydiae Bacteroidetes/Chlorobi Chloroflexi Chrysiogenetes Cyanobacteria Deferribacteres Deinococcus-Thermus Dictyoglomi Fibrobacteres/Acidobacteria Firmicutes Fusobacteria Gemmatimonadetes Lentisphaerae Nitrospirae Planctomycetes Proteobacteria Spirochaetes Thermodesulfobacteria Thermomicrobia Thermotogae Verrucomicrobia Bacteria (singular: bacterium) are unicellular microorganisms. ... Life on Earth redirects here. ...

See also

• Cell signaling

Cell signaling is part of a complex system of communication that governs basic cellular activities and coordinates cell actions. ...

References

1. ^ Chen X, Schauder S, Potier N, Van Dorsselaer A, Pelczer I, Bassler B, Hughson F (2002). "PDF Structural identification of a bacterial quorum-sensing signal containing boron". Nature 415 (6871): 545-9. PMID 11823863. 
2. ^ Cornelis P (editor). (2008). Pseudomonas: Genomics and Molecular Biology, 1st ed., Caister Academic Press. ISBN 978-1-904455-19-6 .