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Purified Recombinant Bluetongue Virus VP1 Exhibits RNA Replicase Activity -- Boyce et al. 78 (8): 3994 -- The Journal ... (5183 words) |
 | Replicase assays were performed with 2 µg of purified VP1 and 2 µg of S10 plus-strand template RNA and various concentrations of magnesium acetate, as indicated. |
| Replicase assays were performed with 2.5 µg of purified VP1 and 2.5 µg of BTV plus-strand RNA produced by in vitro transcription. |
| Template RNA for the replicase assay was produced by in vitro transcription of the construct shown in panel A after linearization with the restriction endonucleases indicated. |
| RNA Virus Replication (3341 words) |
| RNA viruses tend to have a relatively small genome (although virion size may not necessarily be small). |
| RNA replication occurs in the nucleus using a virus-coded enzyme (this may be same as the RNA polymerase involved in transcription of mRNAs, or a modified version). |
| In the cytoplasm, the virion RNA is copied by the viral RNA polymerase while still in a nucleocapsid that has fewer proteins associated with it than are associated with the ISVP or the virion. |
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