RasGAP (Ras GTPase activating protein) is a 120-kDacytosolic protein that provides two principal activities: The Kampfgruppen der Arbeiterklasse (German: Combat Groups of the Working Class) was a paramilitary organisation in East Germany, founded in 1953 and abolished in 1990. ... ÃThe cytosol (as opposed fatty cytoplasm, which also includes the organelles) is the internal fluid of the cell, and a large part of cell metabolism occurs here. ...
Inactivation of Ras from its active GTP-bound form to its unactive GDP-bound form by enhancing the endogenous GTPase activity of Ras, via its C-terminal GAP domain
Mitogenic signal transmission towards downstream interacting partners through its N-terminal SH2-SH3-SH2 domains
Ras (in molecular biology) is the name of a protein, the gene that encodes it, and the family and superfamily to which it belongs. ... Guanosine triphosphate (GTP) is also known as guanosine-5-triphosphate. ... GTPases are a large family of enzymes that can bind and hydrolyze GTP. The GTP binding and hydrolysis takes place in the highly conserved G domain common to all GTPases. ...
Domains
RasGAP contains one SH3 domain and two SH2 domains, a PH domain, and a GAP domain. An SH3 domain is a protein module, a characteristic peptide sequence. ... In structural biology, SH2, or Src homology 2 domain, is a structural domain in signal transduction proteins. ...
RasGAP is a Caspase 3 substrate (1) and acts as both pro- and anti-apoptotic protein depending on the extent of its cleavage by caspases (2).
At low levels of caspase activity, RasGAP is cleaved at position 455, generating an N-terminal fragment (fragment N) and a C-terminal fragment (fragment C).
Fragment N appears to be a general blocker of apoptosis downstream of caspase activation because it inhibits caspase 9-induced cell death.
melanogaster RasGAP was fused in-frame with the GAL4
B, the immunoblot was probed with a mAb directed against RasGAP as a control for the expression and immunoprecipitation of RasGAP in untransfected and transfected COS cells.
RasGAP Interacts with the Aurora Kinase Domain and Inhibits HsAIRK Activity-- Two truncated versions of Aurora were generated in an attempt to locate the region involved in the binding of Aurora to the
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