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Encyclopedia > Representational Difference Analysis
This article may be too technical for most readers to understand. Please expand it to make it accessible to non-experts, without removing the technical details.

Representational Difference Analysis is a technique to find differences in two genomic or cDNA samples. Genomics or cDNA sequences from two samples are PCR amplified and differences analyzed using subtractive DNA hybridization. In genetics, complementary DNA (cDNA) is single-stranded DNA synthesized from a mature mRNA template. ... Wikipedia does not yet have an article with this exact name. ...


  Results from FactBites:
 
EID Vol 2 No 3: Molecular Approaches to the Identification
of Unculturable Infectious Agents (5455 words)
Representational difference analysis is one of the more robust methods of identifying new agents since it does not require prior knowledge of the agent's class (10).
Representational difference analysis depends first on digesting DNA from both healthy and diseased tissues by using a restriction enzyme and then on separately “simplifying” the resulting genomes to reduce their sequence complexity.
A 341-bp representational difference analysis band was isolated from MS tissues with near sequence identity to a region of the major DNA-binding protein gene of HHV-6 (44).
Representational Difference Analysis and Real-Time PCR for Strain-Specific Quantification of Lactobacillus sobrius sp. ... (2048 words)
Representational Difference Analysis and Real-Time PCR for Strain-Specific Quantification of Lactobacillus sobrius sp.
RDA fragments identified after sequencing analysis and BLASTx were as follows: A, a 500-bp fragment predicted to code for a polypeptide with highest similarity (29% at the AA level) to LBA0036, a hypothetical protein from L.
96 and 103% for the primers amplifying the RDA fragment.
  More results at FactBites »


 

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