The Stokes radius or hydrodynamic diameter is the radius of a hydrated atom, but can also be applied to molecules.
It is the effective radius of a molecule as it tumbles rapidly in solution. A more extended molecule will have a larger Stoke's Radii compared to a more compact molecule of the same molecular weight. In liquids where there are considerable interactions between solute and solvent molecule, Stokes radius is proportional to frictional coefficient f and inverse proportional to viscosity η:
r = f / 6πη
Frictional coefficient is determined by the size and shape of the molecule under consideration.
The elution profile of band 3 purified from membranes that had been stripped of its cytoskeleton before solubilization was a broad single peak describing a heterogeneous population of oligomers with a mean Stokesradius of 100 Angstrom.
Whether the conversion of dimer to the form described by a Stokesradius of 100 Angstrom was initiated by removal of cytoskeletal components, alkali-induced changes in band 3 conformation, or alkali-induced loss of copurifying ligands remains unclear.
The ability of the different forms of the protein to bind stilbene disulfonates revealed that the dimer had the highest inhibitor binding affinity, and the form characterized by a mean Stokesradius of 114 Angstrom to have the lowest.
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