Sucrase (EC 3.2.1.10, EC 3.2.1.48) is the enzyme involved in the hydrolysis of sucrose to fructose and glucose. It is secreted by the tips of the villi of the epithilum in the small intestines. Its levels are reduced in response to villi blunting events such as ciliac sprue and the inflammation associated with the disorder. Increases in Pregnancy/Lactation and Diabetes as villi hypertrophy. EC numbers (Enzyme Commission numbers) are a numerical classification scheme for enzymes, based on the chemical reactions they catalyze. ... EC numbers (Enzyme Commission numbers) are a numerical classification scheme for enzymes, based on the chemical reactions they catalyze. ... Ribbon diagram of the catalytically perfect enzyme TIM. An enzyme is a protein that catalyzes, or speeds up, a chemical reaction. ... Hydrolysis is a chemical process in which a molecule is split into two parts by the addition of a molecule of water. ... Sucrose (C12H22O11) is the common chemical name for table sugar. ... Fructose is a simple sugar (monosaccharide) found in many foods and one of the three most important blood sugars along with glucose and galactose. ... Glucose (Glc), a monosaccharide, is one of the most important carbohydrates. ...
CSID is an inherited metabolic disease characterized by a complete or almost complete lack of sucrase and a deficiency of isomaltase actvity.
In the absence of sucrase and isomaltase activity, ingested sucrose (sugar) and isomaltose (type of starch) are not broken down and cannot be absorbed in the gastrointestinal tract.
The deficiency of sucrase activity can be treated by replacing the missing enzyme enabling the products of sucrosehydrolysis, glucose and fructose to be absorbed.
Testing for sucrases optimum environment, multiple reactions were ran using varying amounts and concentrations of sucrose and sucrase at different pHs and temperatures.
Therefore the optimum temperature for sucrase is 37 degrees Celsius, the optimum pH is 2.0, and the optimum concentration is 10%.
For the negative control of 2.5% and 10% sucrase a large test tube was prepared with 3ml of the optimum pH buffer from the previous experiment and exposed to the optimum temperature determined from the previous experiment at time zero.
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