 a Tissue MicroArray Block
 0.6um core Tissue MicroArray Block Tissue microarrays (also TMAs) consist of paraffin blocks in which up to 1000 separate tissue cores are assembled in array fashion to allow simultaneous histological analysis. The major limitations in molecular clinical analysis of tissues include the cumbersome nature of procedures, limited availability of diagnostic reagents and limited patient sample size. The technique of tissue microarray was developed to address these issues. Image File history File links Tissue_MicroArray_Block. ...
Image File history File links Tissue_MicroArray_Block. ...
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Biological tissue is a collection of interconnected cells that perform a similar function within an organism. ...
A thin section of lung tissue stained with hematoxylin and eosin. ...
A reagent is any substance used in a chemical reaction. ...
Multi-tissue blocks were first introduced by H. Battifora in 1986 with his so called “multitumor (sausage) tissue block" and modified in 1990 with its improvement, "the checkerboard tissue block" . In 1998 J. Kononen and collaborators developed the current technique, which uses a novel sampling approach to produce tissues of regular size and shape that can be more densely and precisely arrayed.
 a Tissue MicroArray Section In the tissue microarray technique, a hollow needle is used to remove tissue cores as small as 0.6 mm in diameter from regions of interest in paraffin embedded tissues such as clinical biopsies or tumor samples. These tissue cores are then inserted in a recipient paraffin block in a precisely spaced, array pattern. Sections from this block are cut using a microtome, mounted on a microscope slide and then analyzed by any method of standard histological analysis. Each microarray block can be cut into 100 – 500 sections, which can be subjected to independent tests. Tests commonly employed in tissue microarray include immunohistochemistry, and fluorescent in situ hybridization. Tissue microarrays are particularly useful in analysis of cancer samples. For example, FullMoon Biosystems’ human tissue arrays includes more than 23 different type of tumor tissues, such as brain, breast, lung, kidney, ovary, prostate, thyroid, skin, lymph node, liver, colon, rectum and uterus etc [1]. Image File history File links Tissue_MicroArray_Slide. ...
Image File history File links Tissue_MicroArray_Slide. ...
Histological section refers to thin slices of tissue applied to a microscopic slide, usually around 5 to 10 micrometres thick, which are viewed under a microscope. ...
A microtome is a mechanical instrument used to cut very thin slices for microscopic examination. ...
Immunohistochemistry or IHC refers to the process of localizing proteins in cells of a tissue section exploiting the principle of antibodies binding specifically to antigens in biological tissues. ...
A metaphase cell positive for the bcr/abl rearrangement using FISH. The chromosomes can be seen in blue. ...
Cancer is a class of diseases or disorders characterized by uncontrolled division of cells and the ability of these to spread, either by direct growth into adjacent tissue through invasion, or by implantation into distant sites by metastasis (where cancer cells are transported through the bloodstream or lymphatic system). ...
Superscript text==References==
- Battifora H: The multitumor (sausage) tissue block: novel method for immunohistochemical antibody testing. Lab Invest 1986, 55:244-248.
- Battifora H, Mehta P: The checkerboard tissue block. An improved multitissue control block. Lab Invest 1990, 63:722-724.
- Kononen J, Bubendorf L, Kallioniemi A, Barlund M, Schraml P, Leighton S, Torhorst J, Mihatsch MJ, Sauter G, Kallioniemi OP: Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat Med 1998, 4:844-847.
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See also The MicroArray and Gene Expression (MAGE) group is working on a standard for the representation of microarray expression data that would facilitate the exchange of microarray information between different data systems. ...
Cytomics is the study of cell systems (cytomes) at a single cell level. ...
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