The Upper St. John River Valley is a region in the Canadian province of New Brunswick.

It consists of the communities within the St. John River Valley north of Fredericton and, as such, encompasses northern York County, and nearly the entirety of Carleton and Victoria counties and a small part of southern Madawaska County where the river begins to form the border between Canada and the United States.

The major communties of the valley are Woodstock and Grand Falls.