Helicobacter Pylori Urease drawn from PDB 1E9Z.

Urease (EC 3.5.1.5) is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. The reaction occurs as follows: (NH₂)₂CO + H₂O → CO₂ + 2NH₃ Image File history File links Created on April 10th, 2005 by User:Jag123 using Deepview Description: PDB:1E9Z, Ribbon model of Helicobacter Pylori Urease File links The following pages link to this file: Urease ... Image File history File links Created on April 10th, 2005 by User:Jag123 using Deepview Description: PDB:1E9Z, Ribbon model of Helicobacter Pylori Urease File links The following pages link to this file: Urease ... The Protein Data Bank (PDB) is a repository for 3-D structural data of proteins and nucleic acids. ... The Enzyme Commission number (EC number) is a numerical classification scheme for enzymes, based on the chemical reactions they catalyze. ... Ribbon diagram of the enzyme TIM, surrounded by the space-filling model of the protein. ... In chemistry and biology, catalysis is the acceleration (increase in rate) of a chemical reaction by means of a substance, called a catalyst, that is itself not consumed by the overall reaction. ... Hydrolysis is a chemical reaction or process in which a molecule is split into two parts by reacting with a molecule of water, which has the chemical formula H2O. One of the parts gets an OH- from the water molecule and the other part gets an H+ from the water. ... Urea is an organic compound of carbon, nitrogen, oxygen and hydrogen, with the formula CON2H4 or (NH2)2CO. Urea is also known as carbamide, especially in the recommended International Non-proprietary Names (rINN) in use in Europe. ... Carbon dioxide is a chemical compound composed of one carbon and two oxygen atoms. ... Ammonia is a compound of nitrogen and hydrogen with the formula NH3. ... Urea is an organic compound of carbon, nitrogen, oxygen and hydrogen, with the formula CON2H4 or (NH2)2CO. Urea is also known as carbamide, especially in the recommended International Non-proprietary Names (rINN) in use in Europe. ... Carbon dioxide is a chemical compound composed of one carbon and two oxygen atoms. ... Ammonia is a compound of nitrogen and hydrogen with the formula NH3. ...

In 1926 James Sumner showed that urease is a protein. Urease is found in bacteria, yeast and several higher plants. 1926 (MCMXXVI) was a common year starting on Friday (link will take you to calendar). ... James Batcheller Sumner (November 19, 1887 - August 12, 1955) was an American chemist. ... A representation of the 3D structure of myoglobin, showing coloured alpha helices. ... Phyla/Divisions Actinobacteria Aquificae Bacteroidetes/Chlorobi Chlamydiae/Verrucomicrobia Chloroflexi Chrysiogenetes Cyanobacteria Deferribacteres Deinococcus-Thermus Dictyoglomi Fibrobacteres/Acidobacteria Firmicutes Fusobacteria Gemmatimonadetes Nitrospirae Omnibacteria Planctomycetes Proteobacteria Spirochaetes Thermodesulfobacteria Thermomicrobia Thermotogae Bacteria (singular, bacterium) are a major group of living organisms. ... To meet Wikipedias quality standards, this article or section may require cleanup. ... Divisions Green algae Chlorophyta Charophyta Land plants (embryophytes) Non-vascular plants (bryophytes) Marchantiophyta - liverworts Anthocerotophyta - hornworts Bryophyta - mosses Vascular plants (tracheophytes) †Rhyniophyta - rhyniophytes †Zosterophyllophyta - zosterophylls Lycopodiophyta - clubmosses †Trimerophytophyta - trimerophytes Equisetophyta - horsetails Pteridophyta - true ferns Psilotophyta - whisk ferns Ophioglossophyta - adderstongues Seed plants (spermatophytes) †Pteridospermatophyta - seed ferns Pinophyta - conifers Cycadophyta - cycads Ginkgophyta...

Characteristics:

• Active site metal: nickel(II)
• Molecular weight : 480 kDa or 545 kDa for Jack Bean Urease (calculated mass from the amino acid sequence).
• Optimum pH : 7.4
• Enzymatic specificity: urea and hydroxyurea
• Inhibitors : heavy metal

The multi-subunit enzyme usually has a 3:3 (alpha:beta) stoichiometry with a 2-fold symmetric structure (note that the image above gives the structure of the asymmetric unit, one third of the true biological assembly). An exceptional urease is found in Helicobacter pylori, which combines four of the regular six subunit enzymes in an overall tetrahedral assembly of 24 subunits (α₁₂β₁₂). This supra-molecular assembly is thought to confer additional stability for the enzyme in this organism, which functions to produce ammonia in order to neutralise stomach acid. The active site of an enzyme is the binding site where catalysis occurs. ... The molecular mass of a substance (less accurately called molecular weight and abbreviated as MW) is the mass of one molecule of that substance, relative to the unified atomic mass unit u (equal to 1/12 the mass of one atom of carbon-12). ... The unified atomic mass unit (u), or dalton (Da), is a small unit of mass used to express atomic and molecular masses. ... The unified atomic mass unit (u), or dalton (Da), is a small unit of mass used to express atomic and molecular masses. ... . The initial letter is shown capitalized due to technical restrictions. ... Hydroxyurea chemical structure Hydroxyurea or hydroxycarbamide (rINN), (brand names include Hydrea®) is an antineoplastic drug used in hematological malignancies. ... HIV protease in a complex with the protease inhibitor ritonavir. ... Heavy metals, in chemistry, are chemical elements of a particular range of atomic weights. ... Binomial name Helicobacter pylori ((Marshall 1985) Goodwin 1989) Helicobacter pylori is a bacterium that infects the mucus lining of the stomach and duodenum. ...