| Title of Invention: Assembled viral particles and t heir use in a vaccine to rotaviral disease (8247 words) |
 | VP7 is the major outer shell glycoprotein with an approximate molecular mass of 38 kD in its unreduced form (as determined by SDS-PAGE) and 42 kD in its reduced form (as determined by SDS-PAGE). |
| VP7 is composed of approximately 325 amino acids and the nucleotide sequence and deduced amino acid sequence of VP7 from strain C486 (bovine) is shown in FIG. |
| VP7 and VP4 protein concentrations in growth media and infected cell lysates were estimated on SDS-PGE gels stained with Coomassie Blue, and western blots probed with VP7 and VP4 specific antisera. |
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