Zif268 is a mouse transcription factor that was also named Krox-24, NGFI-A and EGr1. The DNA binding domain of Zif268 consists of zinc finger proteins (ZFPs). The crystal structure of DNA bound by the ZFPs of Zif268 has been solved, which greatly aided ealry research in ZFPs.
Functional serum response elements upstream of the growth factor-inducible gene zif268.The zif268 gene, which encodes a protein with three typical zinc finger sequences, is induced in mouse 3T3 cells by serum, phorbol 12-myristate 13-acetate platelet-derived growth factor, and fibroblast growth factor.
To determine whether the zif268 SRE-like elements mediate induction, CAT (chloramphenicol acetyltransferase) plasmids with different lengths of zif268 upstream sequences were tested for inducibility in 3T3 cells by serum, platelet-derived growth factor, or phorbol 12-myristate 13-acetate.
We conclude that the zif268 SRE-like sequences are functional and probably account for the coordinate induction of zif268 and c-fos.
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