In genetics, splicing is a modification of genetic information prior to translation.
In eukaryotes, a gene often contains altering sequences known as exons and introns. In contrast to prokaryotes, which do not have introns, the primary mRNA transcript called pre-mRNA (see transcription) from the DNA has to be spliced, that means, the introns are removed from the mRNA in an intramolecular reaction, where the mRNA acts as a ribozyme, with the assistance of spliceosomes. The spliceosome also attaches new noncoding units:
A 5' cap, a guanine triphosphate nucleotide, thus named because it binds to the 5' end of the mRNA;
A leader that follows the 5' cap but precedes the exons;
In many cases, the splicing process can create many unique proteins from a large collection of exons. This phenomenon is called alternative splicing.
XXXXEEEEIIIEEEEEEEEEEIIIIEEEEEEEEEEXXXX DNA with exons and introns ↓transcription↓ EEEEIIIEEEEEEEEEEIIIIEEEEEEEEEE mRNA (primary transcript) with exons and introns ↓ splicing ↓ CLLLEEEEEEEEEEEEEEEEEEEEEEEETTTTAAAA mRNA (spliced) with exons, 5' cap, leader, trailer and poly-A tail ↓ translation ↓ polypeptide
The cable splicing techniques described here are for in-building applications using single sheath cable (yet to be activated), and fire-retardant, 25-pair connector modules in a two-bank, in-line configuration.
The splice wrap should be snug, yet not too tight, so that modules and cable pairs aren't lodged in the splice closure seams.
Based on the splice hardware you use and the type of cable you're splicing, determine the proper amount of each cable element to strip (outer jacket, strength members, buffer tube, or subunit jacket) before beginning the splice.
Splice diagrams are graphs decorated with integer labels at each end of each edge and a number of arrows, often none at all, attached to each vertex.
The distinction between a splice diagram and its underlying directed graph, as well as that between a splice diagram vertex and its underlying vertex, is often left implicit.
The regular splice diagram at infinity of the jacobian pencil P. This diagram is considered to be rooted at vertex 1 corresponding to the line at infinity of the ambient plane of P. Its underlying graph is directed away from this root.
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